The composition and expression of Axin mutants. (A) Schematic representation of the mutants of the Axin mutants used in this study. The binding sites for HIPK2, β-catenin and p53 are indicated. (B) BE1 cells were transfected with the indicated EGFP-tagged Axin construct. Total cell lysates were resolved by SDS-PAGE and Western blot analysis was performed using anti-EGFP antibody to detect the expression of the different Axin mutants.