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Figure 4 | Molecular Cancer

Figure 4

From: Axin downregulates TCF-4 transcription via β-catenin, but not p53, and inhibits the proliferation and invasion of lung cancer cells

Figure 4

The effect of transfecting various Axin plasmids into A549 cells. Axin, AxinΔβ-ca, AxinΔp53 and AxinΔβ&P were transfected into A549 cells. (A) Forty-eight hours after transfection total RNA was isolated and RT-PCR analysis was performed using the indicated primers. (B) Quantification of mRNA expression after transfection. (C) Protein expression of A549 cells 48 h after transfection using the indicated antibodies. (D) Quantification of protein expression 48 h after transfection of A549 cells with (E) the TCF-4 promoter construct and (F) TCF reporter construct. * P < 0.05, ** P < 0.01.

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Molecular Cancer

ISSN: 1476-4598