The effect of transfecting various Axin plasmids into A549 cells. Axin, AxinΔβ-ca, AxinΔp53 and AxinΔβ&P were transfected into A549 cells. (A) Forty-eight hours after transfection total RNA was isolated and RT-PCR analysis was performed using the indicated primers. (B) Quantification of mRNA expression after transfection. (C) Protein expression of A549 cells 48 h after transfection using the indicated antibodies. (D) Quantification of protein expression 48 h after transfection of A549 cells with (E) the TCF-4 promoter construct and (F) TCF reporter construct. * P < 0.05, ** P < 0.01.