The effect of LiCl treatment on A549 cells transfected with different Axin plasmids. Axin, AxinΔβ-ca, AxinΔp53 and AxinΔβ&P were transfected into A549 cells. Twenty-four hours post-transfection, cells were treated with LiCl (20 mM). (A) Forty-eight hours after transfection total RNA and RT-PCR analysis was performed using the indicated primers. (B) Quantification of mRNA expression after transfection. (C) Protein expression in A549 cells 48 h after transfection using the indicated antibodies. (D) Quantification of protein expression 48 h after transfection with (E) the TCF-4 promoter construct and (F) TCF reporter construct. * P < 0.05, ** P < 0.01.