The effect of GSK-3β siRNA treatment on BE1 cells and A549 cells transfected with different Axin plasmids. Axin, AxinΔβ-ca, AxinΔP53 and AxinΔβ&P were cotransfected with GSK-3? siRNA into BE1 (A-D) and A549 (E-H) cells. (A&E) Forty-eight hours after transfection total RNA and RT-PCR analysis was performed using the indicated primers. (B&F) Quantification of mRNA expression after cotransfection. (C&G) Western blot analysis of BE1 and A549 cells 48 h after transfection using the indicated antibodies. (D&H) Quantification of protein expression after transfection. * P < 0.05, ** P < 0.01.