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Figure 7 | Molecular Cancer

Figure 7

From: Axin downregulates TCF-4 transcription via β-catenin, but not p53, and inhibits the proliferation and invasion of lung cancer cells

Figure 7

The effect of GSK-3β siRNA treatment on BE1 cells and A549 cells transfected with different Axin plasmids. Axin, AxinΔβ-ca, AxinΔP53 and AxinΔβ&P were cotransfected with GSK-3? siRNA into BE1 (A-D) and A549 (E-H) cells. (A&E) Forty-eight hours after transfection total RNA and RT-PCR analysis was performed using the indicated primers. (B&F) Quantification of mRNA expression after cotransfection. (C&G) Western blot analysis of BE1 and A549 cells 48 h after transfection using the indicated antibodies. (D&H) Quantification of protein expression after transfection. * P < 0.05, ** P < 0.01.

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