Figure 1
From: Trop2 expression contributes to tumor pathogenesis by activating the ERK MAPK pathway
Expression of mTrop2 can promote cell proliferation and cell cycle progression. (a) Stable murine pancreatic adenocarcinoma cells (Panc02) expressing either mTrop2 (Panc02-mTrop2) or GFP (Panc02-GFP) were generated. The increase in mTrop2 mRNA levels in Panc02-mTrop2 cells was measured by quantitative real-time RT PCR. The y axis represents the normalized mTrop2 expression relative to β-actin expression. The protein levels of mTrop2 were determined by Western blot analysis and its membrane incorporation by flow cytometry. (b) Panc02-mTrop2 and control cells were seeded in 96-well plates (1 × 103 cells/well) and serum-starved for 24 h before changing to growth medium containing 0.2% FBS. MTS assay was performed after breaking serum starvation. Relative increase in viability was measured by dividing the viability at one time point by the viability of the same cell at day 0. Absorbance means ± SD (n = 5) are shown. *P < 0.01. (c) Cell cycle analysis. After 24 h of serum starvation, cells were released by the addition of 2% FBS for 4 h (Panc02 and 4T1 cells) or 8 h (MC38 cells). Cells were collected, fixed, stained with propidium iodide and analyzed for cell cycle phase distribution. Data shown is representative from three independent experimental repeats.