Figure 3
From: Trop2 expression contributes to tumor pathogenesis by activating the ERK MAPK pathway
mTrop2 expression increases tumorigenesis in subcutaneous and orthotopic tumor models. (a) Nu/nu mice were inoculated subcutaneously with 2 × 105 Panc02-GFP or Panc02-mTrop2 cells. Tumor size was monitored for 22 days with digital calipers and volumes reported as mean ± SE (n = 5). *P < 0.01. (b) For the orthotopic model, nude mice were inoculated with 5 × 104 Panc02, Panc02-GFP or Panc02-mTrop2 cells in the pancreas. Mice were monitored daily and euthanized after 14 days; data are reported as mean ± SD (n = 6). *P < 0.01. (c) Expression of mTrop2 led to an increase in tumor size and cell proliferation. Immunohistochemistry of paraffin embedded tumor tissues show expression of mTrop2 and Ki-67. (d) Panc02-mTrop2 cell inoculation results in liver metastasis as depicted in the square and blow out picture. (e) H&E staining shows normal liver cell structure in Panc02 and Panc02-GFP inoculated mice and liver metastasis with aberrant liver cell structure in Panc02-mTrop2 inoculated mice (10× low magnification and 40× high magnification) (f) Immunohistochemistry for Ki-67, PCNA and mTrop2 in livers from Panc02 (A-C), Panc02-GFP (D-F) and Panc02-mTrop2 (G-I at 10× and J-L at 20× magnification) inoculated mice. All images shown are representative of group.