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Figure 5 | Molecular Cancer

Figure 5

From: Trop2 expression contributes to tumor pathogenesis by activating the ERK MAPK pathway

Figure 5

mTrop2 expression stimulates the ERK MAPK pathway. (a) Subconfluent cells were used to prepare cell lysates and 60 μg of protein were used for immunoblotting using antibodies against phosphorylated and total ERK. (b) ERK downstream targets like cyclin E, cyclin D1 and CDK2 show increased expression in mTrop2 expressing cells while p27 is downregulated. (c) Increased expression of cyclin D1 and cyclin E was also observed in tumor tissues from mice inoculated with Panc02-mTrop2 cells, when compared to control Panc02 or Panc02-GFP tumor tissues. Images shown are representative of group. (d) Cell lysates from stable human colorectal cancer (HCT-116) and human pancreatic ductal epithelial (HPDE) cells overexpressing human Trop2 were also used for the detection of activated ERK. Increase in hTrop2 mRNA level in HCT-116 and HPDE cells was measured by quantitative real-time RT PCR. The y axis represents the normalized hTrop2 expression relative to β-actin expression.

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