Figure 1From: Malignant transformation in a defined genetic background: proteome changes displayed by 2D-PAGECell culture model for malignant transformation and experimental workflow. The primary human fibroblast cell line BJ was sequentially transduced with retroviruses encoding hTERT, SV40 ER and H-Ras V12 to generate the fully transformed cell line BJ-TER showing the characteristics of cancer cells. Five independent biological replicates were analyzed for BJ, BJ-T, BJ-TE and BJ-TER.Back to article page