Figure 3
From: P-cadherin counteracts myosin II-B function: implications in melanoma progression
Western blot to verify molecularly the activation status of MLC associated with myosin II-B. The upper panel shows the levels of phosphorylated MLC in BLM LIE and BLM P-cad cells in total lysate (TL) and purified by immunoprecipitation. BLM P-cad shows a reduced amount of total phosphorylated MLC. Lower panels show the amount of phosphorylated MLC associated with myosin II-B by co-immunoprecipitation, indicating the decreased activation status of myosin II-B.