Figure 5
Modulation of apoptosis effectors and inhibitors by salirasib. A, B. Representative Western blot of Bcl-XL (A) and Mcl-1 (B) after one day of treatment. Western blot of the independent experiments were quantified by densitometry and then normalized to their respective β-actin (Bcl-XL) or HSP90 (Mcl-1) (n = 6 per group). Data are finally expressed as relative change compared to the control (DMSO) group arbitrarily set at 1. C, D. Quantification of anti-apoptotic genes (C) and pro-apoptotic genes (D) mRNA expression by quantitative PCR after one day of treatment with salirasib (n = 6 per group). Data of the independent experiments were first normalized to RPL19 (internal standard) and then expressed as relative change versus control (DMSO) set arbitrarily at 1 (dotted line) and are presented as mean ± SEM. * P < 0.05, ** P < 0.01 and *** P < 0.001 in treated groups versus control group.