Figure 2From: Kinome profiling of myxoid liposarcoma reveals NF-kappaB-pathway kinase activity and Casein Kinase II inhibition as a potential treatment optionImmunoblotting was used for verification of the results obtained by Pepchip analysis. Band heights were all corresponding to the manufacturer's datasheets (P-Src: 2 bands between 56-61 kDa, Src: 60 kDa, Casein Kinase 2: 42 kDa, P-p65: 65 kDa and p65: 65 kDa). Both cell lines and primary cultures showed phosphorylation of Src and slight variation in amounts of total Src. Casein Kinase 2 and p65 protein were present in all samples in comparable amounts, as is phosphorylated p65, indicating active NF-kappaB signaling.Back to article page