Kinome profiling of myxoid liposarcoma reveals NF-kappaB-pathway kinase activity and Casein Kinase II inhibition as a potential treatment option

Table 3 Top lists of activated kinases and pathways in different conditions

	top five activated kinases	top activated signalling pathway
normal medium condition (including all kinases in the analysis)	ck2a1	NF-kappaB
	lck	Src
	fyn	RAR
	fgr	PPAR
	yes
normal medium condition (leaving out cell cycle related kinases in the analysis)	ck2a1	NF-kappaB
	lck	Src
	fyn	RAR
	camk2b	PPAR
	prkcd
starved medium condition	mapk14	NF-kappaB
	ck2a1	RAR
	akt1	p53
	egfr	G1/S transition of the cell cycle
	erbb2	oxidative stress response

Whereas activated kinases differed between cells grown in normal medium (RMPI supplemented with 10% inactivated calf serum), top five of activated signaling pathways were identical. Results were identical for cell lines and primary cultures. Cell lines cultured in starved medium conditions revealed a different top list of both kinases and activated signaling pathways. However, in all three different conditions, NF-kappaB was the most activated signaling pathway identified.
prkcd: protein kinase C, delta; mapk14: mitogen-activated protein kinase 14; egfr: epidermal growth factor receptor; erbb2: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2

ISSN: 1476-4598