Table 1 Genes most responsive to Runx2 in C4-2B cells
From: Runx2 transcriptome of prostate cancer cells: insights into invasiveness and bone metastasis
Gene ID | Symbol | Gene Name | Anova | Fold change | Description of cancer related or other major function | Reference | ||
|---|---|---|---|---|---|---|---|---|
| Â | Â | Â | p value | I | II | q | Â | Â |
Transcription regulators | ||||||||
7069 | THRSP | Thyroid hormone responsive | 3.5E-06 | 7 | 5 | Â | Transcription of enzymes involved in tumor lipid metabolism | Â |
29947 | DNMT3L | DNA (cytosine-5-)-methyltransferase 3-like | 5.5E-08 | 5 | 6 | > 50 | DNA methylation, nucleic acid metabolic process | Â |
4091 | SMAD6 | SMAD family member 6 | 8.1E-10 | 5 | 4 | Â | Cell communication, TGF-beta receptor signaling pathway | Â |
6591 | SNAI2 | Snail homolog 2 (Drosophila) | 8.8E-07 | 4 | 12 | 6 | Promotes EMT by repressing E-cadherin transcription | [44] |
6662 | SOX9 | SRY (sex determining region Y)-box 9 | 4.4E-07 | 4 | 6 | 17 | Promotes chondrocyte differentiation, cell migration, and EMT | [110] |
7025 | NR2F1 | Nuclear receptor subfamily 2, | 3.5E-07 | 3 | 14 | 2 | Ligand-dependent receptor, regulation of transcription termination | Â |
678 | ZFP36L2 | Zinc finger protein 36, C3H type-like 2 | 4.1E-09 | 3 | 10 | Â | Regulation of RNA metabolic process | Â |
4088 | SMAD3 | SMAD family member 3 | 6.3E-10 | 3 | 7 | 25 | Signal transducer downstream of TGF-β receptors |  |
283248 | RCOR2 | REST corepressor 2 | 2.9E-06 | 3 | 7 | Â | Negative regulation of transcription | Â |
10217 | CTDSPL | Small CTD phosphatase 3 | 1.9E-09 | -2 | -3 | Â | Phosphatase, dephosphorylates Rb, suppresses cellular growth | Â |
1870 | E2F2 | E2F transcription factor 2 | 8.2E-10 | -2 | -5 | -4 | Transcription activator, regulation of cell cycle progression, proliferation | Â |
90390 | MED30 | Mediator complex subunit 30 | < 1.0E-12 | -2 | -3 | Â | Transcription activator, Androgen receptor signaling pathway | Â |
4609 | MYC | V-myc myelocytomatosis viral oncogene | 2.9E-07 | -2 | -3 | -2 | Positive regulation of cell proliferation, apoptosis | |
29893 | PSMC3IP | PSMC3 interacting protein | 3.5E-08 | -2 | -4 | Â | Gene expression from RNA PolII | Â |
4602 | MYB | Viral oncogene homolog (avian) | 1.6E-08 | -3 | -5 | -3 | G1/S transition of mitotic cell cycle | Â |
4824 | NKX3-1 | NK3 homeobox 1 | 3.7E-08 | -3 | -3 | Â | Transcription repressor, loss induces prostate tumorigenesis | Â |
Intracellular signaling, cell cycle and proliferation | ||||||||
51655 | RASD1 | RAS, dexamethasone-induced 1 | 6.8E-12 | 26 | 19 | > 50 | Ras related, suppresses cell proliferation, inhibits tumor growth | [92] |
6446 | SGK | Serum/glucocorticoid regulated kinase 1 | < 1.0E-12 | 19 | 45 | Â | Cellular Na+ ion homeostasis, cell survival | Â |
57007 | CXCR7 | Chemokine receptor 7 | 9.2E-11 | 8 | 12 | 30 | Receptor for SDF-1, GPCR, enhanced integrin activation and tumor growth | [113] |
1843 | DUSP1 | Dual specificity phosphatase 1 | 9.1E-13 | 8 | 8 | 10 | Regulator of cell cycle, response to stress | [114] |
5580 | PRKCD | Protein kinase C, delta | 2.0E-11 | 6 | 5 | 12 | Serine-Threonine Kinase, cell proliferation, cell contraction | Â |
55890 | GPRC5C | G protein-coupled receptor, family C | 1.3E-09 | 5 | 5 | 25 | GPCR, cell communication | Â |
10461 | MERTK | C-mer proto-oncogene tyrosine kinase | 6.2E-10 | 4 | 6 | 39 | Protein amino acid phosphorylation | Â |
5920 | RARRES3 | Retinoic acid receptor responder 3 | 4.3E-08 | 3 | 8 | 40 | Negative regulation of cell proliferation | Â |
1903 | EDG3 | Sphingosine-1-phosphate receptor 3 | 4.8E-10 | 3 | 7 | Â | GPCR, enhances tumor growth, cell migration, and angiogenesis | |
8877 | SPHK1 | Sphingosine kinase 1 | 7.5E-09 | 3 | 12 | 36 | Kinase for the production of S1P, enhances tumor growth, cell migration, and angiogenesis, PCa cell survival against chemotherapy, osteoclast chemotaxis | |
7049 | TGFBR3 | Transforming growth factor, β receptor III | 7.1E-09 | 3 | 4 | 12 | Negative regulation of TGFβ receptor signaling pathway |  |
4921 | DDR2 | Discoidin domain receptor tyrosine kinase 2 | 2.9E-05 | 2 | 5 | Â | Collagen binding, cell proliferation | Â |
1017 | CDK2 | Cyclin-dependent kinase 2 | 1.3E-03 | -2 | -3 | -4 | Kinase, mitotic cell cycle, cell proliferation | Â |
55038 | CDCA4 | Cell division cycle associated 4 | 7.2E-08 | -2 | -4 | -4 | Repressor of E2F-dependent transcriptional activation and cell proliferation | Â |
5997 | RGS2 | Regulator of G-protein signaling 2, 24 kDa | 9.0E-09 | -3 | -3 | Â | Cell cycle, GPCR signaling pathway, cell communication | Â |
25805 | BAMBI | BMP and activin membrane-bound inhibitor homolog (Xenopus laevis) | 9.7E-10 | -2 | -3 |  | TGF-β signaling pathway |  |
Cellular movement, adhesion, invasion, cytoskeleton remodeling | ||||||||
89797 | NAV2 | Neuron navigator 2 | 6.7E-09 | 9 | 10 | 29 | Scaffold protein, actin cytoskeleton remodeling, nervous system development | [53] |
27111 | SDCBP2 | Syndecan binding protein 2 | 2.2E-03 | 6 | 15 | Â | Binds SDC2, regulates cell adhesion and communication with ECM molecules | |
11344 | PTK9L | Twinfilin | 9.0E-10 | 6 | 5 | 11 | Actin-binding protein, cytoskeleton organization | [57] |
83715 | ESPN | Espin | 2.1E-08 | 5 | 8 | > 50 | Actin-binding-bundling protein, actin cytoskeleton dynamics, cell locomotion | |
51474 | LIMA1 | Eplin, LIM domain and actin binding 1 | 3.0E-10 | 4 | 5 | 15 | Cytoskeleton-associated protein, regulation of actin filament depolymerization | [56] |
8829 | NRP1 | Neuropilin 1 | 4.1E-08 | 4 | 11 | 40 | TM, coreceptor for VEGF, collagen binding, cell adhesion, invasion | [119] |
4582 | MUC1 | Mucin 1, cell surface associated | 1.1E-09 | 4 | 4 | Â | TM, cell adhesion, invasion | [120] |
4131 | MAP1B | Microtubule-associated protein 1B | 7.1E-09 | 3 | 9 | 18 | Trans-membrane glycoprotein, cytoskeleton organization | |
9644 | SH3PXD2A | SH3 and PX domains 2A | 1.4E-09 | 3 | 7 | Â | Adaptor protein, ECM degradation by actin based invadopedia formation | [122] |
6275 | S100A4 | S100 calcium binding protein A4 | 2.6E-08 | 2 | 24 | > 50 | EF-hand calcium-binding protein, promotes cell motility, tumor metastasis | [49] |
6383 | SDC2 | Syndecan 2 | 3.1E-06 | 2 | 5 | 32 | TM, bind signaling factors, cell adhesion, angiogenesis, ECM degradation | |
1013 | CDH15 | Cadherin 15, type 1, M-cadherin | 6.9E-08 | 2 | 5 | 21 | TM, Ca2+dependent intercellular adhesion, skeletal muscle development | [123] |
Secreted factors | ||||||||
1437 | CSF2 | Colony stimulating factor 2/GM-CSF | 2.3E-09 | 12 | 17 | > 50 | Cytokine, osteolytic bone metastasis | [72] |
5225 | PGC | Progastricsin (pepsinogen C) | 3.9E-12 | 11 | 15 | Â | Aspartyl proteinase | [47] |
5304 | PIP | Prolactin-induced protein | < 1.0E-12 | 10 | 79 | > 50 | Aspartyl proteinase, ligand for CD4 receptor T cells | [46] |
8857 | FCGBP | Fc fragment of IgG binding protein | 1.5E-09 | 5 | 9 | Â | Cell adhesion, cell-cell recognition | Â |
6387 | CXCL12 | Chemokine ligand 12 (SDF-1) | 2.2E-06 | 5 | 7 | 23 | Chemokine, stem cell trafficking to bone, migration, metastasis of tumor cells | |
7422 | VEGFA | Vascular endothelial growth factor A | 4.5E-10 | 5 | 3 | 4 | Growth factor, angiogenesis, cellular morphogenesis during differentiation | |
1907 | EDN2 | Endothelin 2 | 4.3E-07 | 5 | 2 | Â | Growth factor, vasoactive, G-protein signaling, smooth muscle contraction | [87] |
5654 | HTRA1 | HtrA serine peptidase 1 | 6.9E-10 | 3 | 18 | Â | Serine protease, differentially expressed in osteoarthritic cartillage | [124] |
3817 | KLK2 | Kallikrein-related peptidase 2 | 1.5E-08 | 4 | 3 | Â | Serine protease, protein metabolic process | Â |
4880 | NPPC | Natriuretic peptide precursor C | 1.8E-10 | 4 | 17 | > 50 | Peptide, potent natriuretic, diuretic, and vasodilating | Â |
7057 | THBS1 | Thrombospondin 1 | 3.0E-10 | 4 | 14 | > 50 | Cell adhesion, heparin-binding, anti-angiogenic | Â |
3934 | LCN2 | Lipocalin 2 | 5.1E-08 | 4 | 10 | 5 | ECM protein, present ligands to cell surface receptors, promotes EMT | [45] |
6462 | SHBG | Sex hormone-binding globulin | 8.7E-10 | 4 | 10 | Â | Steroid-binding, signal | Â |
57642 | COL20A1 | Collagen, type XX, α 1 | 4.9E-07 | 4 | 10 | > 50 | Adhesion, establishment of localization, transport |  |
4254 | KITLG | KIT ligand | 2.3E-08 | 4 | 7 | 41 | Hematopoietic growth factor and ligand for the KIT tyrosine kinase receptor | Â |
4884 | NPTX1 | Neuronal pentraxin I | 2.5E-07 | 3 | 20 | Â | Cell-cell signaling, transport, establishment of localization | Â |
3486 | IGFBP3 | IGF binding protein 3 | 2.2E-08 | 3 | 7 | Â | Regulation of cell growth, communication, apoptosis | Â |
9806 | SPOCK2 | Sparc/osteonectin, cwcv, kazal-like domains proteoglycan (testican) 2 | 1.1E-06 | 3 | 4 | Â | Cell differentiation, ECM organization and biogenesis | Â |
8530 | CST7 | Cystatin F (leukocystatin) | 3.0E-09 | 2 | 25 | Â | Competitive inhibitors of cysteine proteases, metastasis associated protein | [48] |
4885 | NPTX2 | Neuronal pentraxin II | 7.6E-09 | 2 | 6 | Â | Cell-cell signaling, synaptic transmission | Â |
147381 | CBLN2 | ADAM MMP with Thbs-1 motif | 5.2E-12 | -3 | -3 | Â | Peptidase, cleavage of precerebellin in the nervous system | Â |
6781 | STC1 | Spondin 2 | 3.6E-08 | -3 | -3 | Â | ECM protein, integrin ligand, pattern recognition | Â |
7035 | TFPI | Tissue factor pathway inhibitor | 3.9E-05 | -3 | -4 | Â | Kunitz-type inhibitor of blood coagulation by blocking factor Xa | Â |
9518 | GDF15 | Growth differentiation factor 15 | 1.6E-09 | -2 | -8 | -7 | Growth factor, TGF-β signaling pathway, cell communication |  |