Skip to main content
Figure 2 | Molecular Cancer

Figure 2

From: Down-regulation of the Nucleotide Excision Repair gene XPG as a new mechanism of drug resistance in human and murine cancer cells

Figure 2

NER Activity in sensitive and resistant L1210 cells. Panel A. Host cell reactivation assay: L1210 parental cells (black circle), L1210/MMDX cells (black square). Panel B. Western blot analysis for the expression of NER-belonging genes ERCC1, XPA and XPG in parental (wt), nemorubicin resistant L1210 cells (MMDX), L1210/0 and L1210/DDP cells. Panel C. Western blot analysis for the expression of XPG in parental (L1210), nemorubicin resistant L1210 cells (MMDX), and in L1210/MMDX clone 5 (CL5) and clone 6 (CL6). Panel D. In vitro growth inhibition induced by nemorubicin in L1210 parental (black circle), L1210/MMDX (black square) cells and in two clones derived from L1210/MMDX (clone 5, (white triangle) clone 6 (white rhomb) ) in which the human XPG gene has been transfected. The values are the mean +/- SD of three replicates performed in three independent experiments. Panel E. Expression of XPG mRNA in L1210 parental and in L1210/MMDX cells measured by Real Time RT-PCR.

Back to article page

Molecular Cancer

ISSN: 1476-4598