Figure 5

In vitro and in vivo activity of nemorubicin in sensitive and resistant HCT116 cells. Panel A. Expression of XPG mRNA in HCT116 parental and five nemorubicin-resistant cells measured by Real Time RT-PCR. Results are reported as decrease over untreated cells and are the mean and SD of at least three replicates. Panel B. In vitro cytotoxic activity of nemorubicin in parental HCT116 (black circle) and M23-resistant (black square) cells without (left) or with (right) 100 nM AZA. Panel C. In vivo activity of nemorubicin in mice transplanted with parental HCT116 (left) or the M23-resistant clone (right). Data are reported as tumor volume determined by measurement of the tumor diameters for untreated (black circle) or nemorubicin-treated (black square) mice. Each group consisted of 8 animals and each point represents the mean +/- SD. * p < 0.01 relative to untreated animals.