Figure 3

Effects of CRF on the expression of molecules involved in tumor growth and metastasis. Gene-specific oligo microarrays for 113 genes known to be involved in tumor growth and metastasis were performed as described in Materials and methods. Effect of CRF on the expression of these molecules in 4T1 cells was analyzed. Mean expression of each gene was transformed into color intensity using the program TIGR MultiExperiment Viewer V4.5.1. The minimal value (green) and the maximum (red) were considered the limits (as noticed in bar above). The figure shows the molecules which expression was affected by CRF after 6 or 24 h of treatment: Bai1, Brain-specific angiogenesis inhibitor 1; Brms1, Breast cancer metastasis-suppressor 1; Ctnnb1, β-Catenin; Cdkn2a, Cyclin-dependent kinase inhibitor 2A; Smad2, MAD homolog 2; Hras1, Harvey rat sarcoma virus oncogene 1; Pten, Phosphatase and tensin homolog; Rb1, Retinoblastoma 1; Rhoc, Ras homolog gene family, member C.