CRF promotes migration of 4T1 cells and expression of Cox-2. A. 4T1 cells were stimulated with 10-8M CRF or vehicle (control) in the presence or absence of Antalarmin and photographed at 0, 6, 12 and 24 hours after disruption of a small area of the cell layer. The remigration of the cells was quantified by measuring the distance at 3 at least different points of the gap of each image with the program Image J, and expressing the distance % of the average of the distance initially formed by the disruption (time 0). Results represent the average of three independent experiments. ++p < 0,01, +++p < 0.001 compared to control cells at the same time point; **p < 0,01; ***p < 0,001 compared to CRF-treated cells at the same time point. B. Expression of Cox-2 was measured in 4T1 stimulated cells by RT-PCR (upper panel) and real-time RT-PCR. Results represent the average of 3 independent experiments; ***p < 0,001 compared to control cells.