Figure 3

Loss of Sin3A increases apoptosis but does not affect the cell cycle of breast cancer cells. MCF7 cells were transfected with scrambled negative control or Sin3A siRNA and treated with vehicle ethanol or 10 nM estrogen (E2) for 72 or 96 hours. Samples were analyzed simultaneously by flow cytometry for (A) cell cycle distribution and (B) annexin V, indicative of apoptotic cells. Data are from three independent experiments with error bars showing the standard error of the mean. To determine statistical significance of the findings in (B), student's t tests were performed comparing corresponding scrambled and Sin3A siRNA samples, *p < 0.05.