Skip to main content
Figure 5 | Molecular Cancer

Figure 5

From: Frequent epigenetic inactivation of RASSF2 in thyroid cancer and functional consequences

Figure 5

Binding studies of RASSFs, MST1 and WW45 in co-precipitation. Plasmids, that express the indicated GST-Flag-tagged-constructs (-GF) and Flag-tagged-constructs (-F) were transfected into HEK293T cells. After two days, total protein was extracted and Flag-tagged proteins were precipitated with anti-Flag-agarose (F) or glutathione sepharose (G). Samples were separated on a 10% PAGE gel and blotted. The precipitated and co-precipitated proteins were determined with anti-Flag-antibodies. The sizes of a protein marker (M) are indicated.

Back to article page