Figure 6

5-aza-deoxydytidine activates ALCAM expression in tumor cells. A) top panel; Histogram showing baseline methylation at each CpG sites in the ALCAM promoter in MDA-MB-435 cells (blank columns) and the percent methylation following treatment with 5 μM 5-aza-deoxycytidine (filled columns). Lower panel; Profile of DNA demethylation across the proximal ALCAM promoter achieved by treating MDA-MB-435 cells with 5 μM 5-aza-deoxycytidine. B) Dose-dependent re-activation of ALCAM mRNA and protein expression in MDA-MB-435 cells treated with a concentration range (0-10 μM) of 5-aza-deoxycytidine.