Inhibitor studies further determine that the IL-6/STAT3 pathway is involved in invasion. A) qRT-PCR demonstrating decreased expression of Stat3 in DU145 shSOX1 clone #7 cells and Mcl-1, a Stat3 target gene. No change was observed in Myc or Survivin. *denotes statistical significant p < 0.05 compared to the vector transfected line. B) Prostatospheres were generated by culturing LNCaP cells in SCM+KO for 7 days and qRT-PCR analysis was performed. Compared to adherent LNCaP cells, expression of Bmx, Sox1 Mcl-1, Myc, Survivin, and Stat3 was significantly increased in the stem-like prostatospheres. *denotes statistical significant p < 0.001 compared to the adherent cells. C) Correlation of Sox1 and Stat3 was analyzed in Oncomine 4.2 (Ann Arbor, MI). The heat map represents raw data from the Varambally over-expression in prostate cancer analysis comparing primary tissue and metastatic tissue. Expression is in terms of normalized over-expression units. The P-value represents a student's t-test comparing primary and metastatic expression. D) Using the GEO database, expression of Sox1 and Bmx were compared between benign, primary or metastatic prostate tissue and significant differences were observed in Sox1. For Stat 3, a comparison between Prostate Intraepithelial Neoplasia (PIN) and invasive prostate tumor tissue yield a significant difference in expression. *denotes statistical significant p < 0.05 compared to either benign samples or PIN.