Figure 2

Therapeutic efficacy of pG8- FasL in primary GBM. (A) Images showed the immunostaining against Ki67 (top panel) and GFAP (middle panel) in primary human glioma cells Bottom panel, primary human glioma cells were infected with MOI of 0.8 of pG8-18. Images showed the percentage of eGFP+ cells. Original magnification ×100 was shown. (B) The percentage of apoptotic cells in pG8-FasL and pG8-18-transduced primary glioma cells. Data shown are the averages of triplicate experiments + SEM. (C) Fluorescence images showed eGFP+ and TUNEL+ cells on pG8-FasL and pG8-18-transduced primary glioma cells 72 h after infection. Transduction efficiency of primary human glioma cells as visualized by eGFP expression. Original magnification ×100 was shown. Images were captured using Nikon TE300 wide-field microscope equipped with a digital color CCD camera. (D) The effect of FasL and FADD expression on primary human glioma cells was examined. The percentage of cell death in primary glioma cells infected with the various amplicon viral vectors was determined after 72 h. Data shown are averages of triplicate experiment + SEM.