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Figure 4 | Molecular Cancer

Figure 4

From: FasL and FADD delivery by a glioma-specific and cell cycle-dependent HSV-1 amplicon virus enhanced apoptosis in primary human brain tumors

Figure 4

Effect of TMZ and IR on gene expression mediated by pG8-18-based vectors. (A) ΔGli36 cells were infected with either pG8-18 or pIH8GalLuc vector for 6 h prior to treatment with TMZ. Cells were treated with TMZ for 1 h, after which the cells were replenished with fresh medium without TMZ and harvested at 48 h post-TMZ treatment for the analysis of luciferase expression. (B) To determine the effect of IR on the cell cycle-regulated transgene expression, ΔGli36 cells were transfected with either pG8-18 or pIH8GalLuc vector for 6 h prior to 3Gy of irradiation, after which the cells were replenished with either fresh medium or medium containing lovastatin. Cells were harvested at 48 h post-transfection for the analysis of luciferase expression. (C) ΔGli36 cells infected with pG8-FasL and pG8-FADD were subjected to 75 μM TMZ, 3 Gy IR, or combination of TMZ and IR. Percentage of cell death was determined after 72 h. Data shown are % cell death in either pG8-18-infected or pG8-FasL/FADD- infected cells treated with TMZ, IR or TMZ and IR. For all experiments, data shown are averages of triplicate experiments + SEM and the total amplicon viruses used was MOI of 1.0.

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