Figure 6

SerpinE2 silencing modulates migration, invasion and adhesion of colorectal cancer cells. A- Migration of shTGFP- and shSerpinE2(#15 or #16)-expressing LoVo cells to the undersurface of the polycarbonate membrane of Boyden chambers coated with fibronectin (FN) or vitronectin (data not shown) was evaluated 24 h after seeding, in presence of 20 mM hydroxyurea. The number of cells was counted in five fields and the experiments were performed in triplicate. The number of shTGFP cells that had migrated was set at 100%. B- Invasion of shTGFP- and shSerpinE2(#15 or #16)-expressing LoVo cells was studied using Matrigel-coated Transwells during 48 h. Thereafter, cells were fixed and stained with DAPI solution. The number of cells was counted in five fields and the experiments performed in duplicate. The number of shTGFP cells which had migrated was set at 100%. C- Time required for complete de-adhesion of shTGFP- and shSerpinE2(#16)-expressing LoVo cells after addition of trypsin while rocking at 100 rpm. *, significantly different from shTGFP at p < 0.05 (Student's t test). ***, significantly different from control at p < 0.0001 (Student t test).