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Figure 5 | Molecular Cancer

Figure 5

From: Macrophages may promote cancer growth via a GM-CSF/HB-EGF paracrine loop that is enhanced by CXCL12

Figure 5

Proliferation induced by supernatants from CXCL12-stimulated mononuclear phagocytes. (A) Human mononuclear phagocytes (Mø) or neutrophils (PMN) were stimulated with 200 ng/mL CXCL12 and cell free supernatants were collected after 24 hours and added to either HeLa or DLD-1 cells. (B) Supernatants from CXCL12-stimulated PMN (SN1) were not effective at inducing phosphorylation of HER1, because PMN did not produce HB-EGF. Supernatants from CXCL12-stimulated Mø (SN2) induced HER1 phosphorylation at Y1068 when added to HeLa or DLD-1 cells (p < 0.05). The phosphorylation was genuinely inhibited by 100 μg/mL anti-HB-EGF neutralising Abs. (C) SN2 caused HeLa and DLD-1 cells to proliferate to a degree that was comparable to stimulation with 25 ng/mL HB-EGF. The means ± SD of 10 experiments are depicted.

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