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Figure 3 | Molecular Cancer

Figure 3

From: Synergistic epigenetic reactivation of estrogen receptor-α (ERα) by combined green tea polyphenol and histone deacetylase inhibitor in ERα-negative breast cancer cells

Figure 3

Alteration of histone modulation of the ERα promoter and histone acetylation-related enzymatic activities. A) Histone modification patterns were analyzed by ChIP assay. Representative photograph from an experiment was repeated in triplicate. B) Histone modification enrichment in the ERα promoter was calculated from the corresponding DNA fragments amplified by ChIP-PCR as shown above. MDA-MB-231 cells were treated as described previously and analyzed by ChIP assays using chromatin markers including acetyl-H3, acetyl-H3K9, acetyl-H4, dimethyl-H3K4, trimethyl-H3K9 and mouse IgG control in the promoter region of ERα. MCF-7 cells served as a positive control. Inputs came from the total DNA and served as the same ChIP-PCR conditions. DNA enrichment was calculated as the ratio of each bound sample divided by the input while the untreated MDA-MB-231 control sample is represented as 1.0. Columns, mean; Bars, SD. C) and D) Histone acetyltransferase (HATs) and Histone deacetylases (HDACs) activities in MDA-MB-231 cells. Nuclear proteins of MDA-MB-231 cells were extracted after the treatment as described above. The HATs and HDACs activity assays were performed according to the manufacturer's protocols. The values of enzymatic activities of HATs and HDACs are the means of three independent experiments. Columns, mean; Bars, SD; *, P < 0.05, * * P < 0.001, significantly different from control.

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