Figure 4

Analysis of response of NRP-1 to multiple HDAC inhibitors. The extent of the concomitant response of Sp1 acetylation and NRP-1 down- regulation was determined using a high-content analysis approach. HCT116 cells were treated for 24 hr with concentration ranges of 0-20 mM sodium butyrate, 0-20 μM Oxamflatin, 0-20 μM Scriptaid, 0-20 μM APHA compound 8, 0-20 μM CHAHA. Cells were stained for acetyl-Sp1 and NRP-1 as described in the methods section. Levels of protein are expressed in terms of fluorophore fluorescence relative to that observed in untreated cells, left column shows fluorescence for acetyl-Sp1 and right column for NRP-1.