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Figure 6 | Molecular Cancer

Figure 6

From: Pim-selective inhibitor DHPCC-9 reveals Pim kinases as potent stimulators of cancer cell migration and invasion

Figure 6

Pim kinase overexpression promotes migration of prostate cancer cells. PC-3 cells were transiently transfected with an empty vector (Mock) or vectors expressing V5-tagged Pim-1, Pim-2 or Pim-3. DMSO or 10 ÎĽM DHPCC-9 were added 24 h after transfection. The wound healing assays were initiated 2 h later by scratching the wounds with a sterile 200 ÎĽl pipette tip. (A) Shown are representative pictures from three time-points. (B) Graph represents means of triplicate samples. (C) Levels of overexpressed Pim kinases were determined by Western blotting with anti-V5 antibody. GAPDH staining was used as a loading control.

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