Figure 6

In vivo blood clearance rate and tumor-targeting of ¹²⁵I-SLT-1A^IYSNKLM (A) Blood clearance of ¹²⁵I-SLT-1A^IYSNKLM shown as percentage of injected dose per mL blood collected at various time points over a 72 h period post-i.v. injection. The clearance rate (t_1/2) of ¹²⁵I-SLT-1A^IYSNKLM was shown to be ~11 min. (B) Biodistribution per collected gram of wet tissue of ¹²⁵I-SLT-1A^IYSNKLM after 1 h (black bars), 6 h (light grey bars), 12 h (dark grey bars) and 24 h (white bars) post-i.v. administration. (C) Tumor localization of ¹²⁵I-SLT-1A^IYSNKLM versus wt ¹²⁵I-SLT-1A (negative control) as shown by nanoSPECT and CT imaging. The composite images show the tissue uptake of the radiolabeled proteins (as pink colored areas) as well as the location of tumor xenografts (white circles) and that of kidneys (grey arrows).