Well known differentiation marker analysis after recombinant IGFBP-rP1 stimulation. The experiments were conducted in three colon cancer cell lines: RKO, SW620, CW2. After attachment for 24 hr, the cells were then stimulated with (-pr, for RKO cells, 10 μg/ml, For SW620 and CW2 cells, 4 μg/ml) or without recombinant IGFBP-rP1 protein (-bl) in medium without FBS. Forty-eight hours after IGFBP-rP1 addition, expression of differentiation markers were performed in the cell lysates and supernatants. A:Total cell lysates and supernatants were harvested and assayed for AKP activity assessing. Results represent the mean (± SD) of three experiments.*p < 0.05. B. Total cell lysates were harvested and assayed for CEA, CDX2 expression. Also shown are the corresponding reprobings of these blots with actin as a loading control. This blot is representative of those obtained when the experiment was performed six times.