Verification of the differentially expressed genes in SW620-IGFBP-rP1 transfectants and CW2-IGFBP-rP1 transfectants. The expression of the 16 reproducible genes in 3 clones and the differentially-expressed cytoskeleton associated genes keratin 7, keratin 8, profilin2 analysed by chip were also analysed by rtRT-PCR in IGFBP-rP1-SW620 and IGFBP-rP1-CW2 transfectants versus empty vector control cells. RtRT-PCR was performed on RNA extracted from the transfectants and the control cells. Delta-delta Ct method was used to calculate relative fold changes normalized against the GAPDH gene. Results represent the mean (± SD) of six experiments.