Figure 5

Effect of MLL knockdown on cellular resistance, metabolism and GC signaling. (A) Effect of gamma-irradiation (γ-IR, 1 Gy), methotrexate (MTX, 0.01 μg/ml), cytarabine (ARAC, 0.025 μg/ml) on growth of MLL-Scr and MLL-KD cell lines over two days; (B) Percentage of dying or necrotic cells (normalized to untreated) in MLL-Scr and MLL-KD after 48 hours in the presence of dexamethasone (DEX, 250 μM or 500 μM) or following gamma-irradiation (γ-IR, 1 Gy); (C) Ratio of lactate production/glucose consumption over two days in MLL-Scr and MLL-KD cells; (D) Relative expression of GILZ mRNA as measured by qRT-PCR in MLL-Scr and MLL-KD cell lines incubated for four hours in the absence (-) or presence (+) of dexamethasone (1 μM); In each case (A-D) data represent mean ± SEM from 3-6 independent experiments with statistical analysis by unpaired t-test; * p < 0.05, ** p < 0.01.