Effects of C3G on ethanol-mediated phosphorylation of ErbB2, cSrc, FAK and p130Cas. A: MCF7ErbB2 cells were treated with ethanol (0 or 400 mg/dl) with/without C3G (10, 20 or 40 μM) for 48 h. Cells were seeded on fibronectin-coated culture wells for 3 h and then harvested for analysis of the phosphorylation of ErbB2, FAK, p130Cas and cSrc with immunoblotting. The expression of actin served as a loading control. B: The relative expression of phosphorylated ErbB2, FAK, p130Cas and cSrc was determined by densitometry and normalized to the expression of actin. * denotes a statistically significant difference from untreated controls. # denotes a significant difference from ethanol-treated groups. ε denotes a significant difference from ethanol- and C3G (10 μM)-treated groups. δ denotes a significant difference from ethanol- and C3G (20 μM)-treated groups. C: The phosphorylation of ErbB2 and FAK in MDA-MB231 cells was analyzed as described above. The experiment was replicated three times.