Effects of C3G on ethanol-induced activation of JNKs. MCF7ErbB2 cells were treated with ethanol (0 or 400 mg/dl) with/without C3G (10, 20 or 40 μM) for 48 h. Cells were seeded on fibronectin-coated culture wells for 3 h. A: Cell lysates were collected and analyzed for the phosphorylation/expression of JNKs with immunoblotting. B: Cell lysates were IP with an anti-JNK antibody and IB with either an anti-p130Cas or anti-JNK antibody. The experiment was replicated three times. C and D: The phoshorylation of JNKs and the association between JNKs and p130Cas were quantified by densitometry. * denotes a statistically significant difference from untreated controls. # denotes a significant difference from ethanol-treated groups. ε denotes a significant difference from ethanol- and C3G (10 μM)-treated groups. δ denotes a significant difference from ethanol- and C3G (20 μM)-treated groups.