Figure 6
From: Cyanidin-3-Glucoside inhibits ethanol-induced invasion of breast cancer cells overexpressing ErbB2
Effects of C3G on the development of lamellipodia. MDA-MB231 cells were treated with ethanol (0 or 400 mg/dl) with/without C3G (10 or 40 μM) for 48 h. Cells were seeded on fibronectin-coated coverslips for 3 h. A: The expression of actin (Alexa Fluor 488 Phalloidin) and phosphorylated FAK (Tyr 861) (Alexa Flour 594) were detected with immunofluorescent staining. The arrow indicates lamellipodia. Scale bar = 5 μm. B: Cells with extended leading areas (lamellipodia) were counted in ten randomly selected fields in each treatment group. The percentage of cells with lamellipodia was determined. The experiment was replicated three times. * denotes a significant difference from untreated controls. # denotes a significant difference from ethanol-treated groups.