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Figure 1 | Molecular Cancer

Figure 1

From: Podoplanin in cancer cells is experimentally able to attenuate prolymphangiogenic and lymphogenous metastatic potentials of lung squamoid cancer cells

Figure 1

Effect of podoplanin expression in tumor cells on cellular function in vitro. A) RT-PCR for human podoplanin and GAPDH was performed with cDNA from LSCC cell lines QG56, H157, and EBC-1 as described in Methods. B) Cell lysates from cultured stable clones indicated were subjected to Western blot analysis for human podoplanin and subsequently to re-probing for β-actin. Exogenous podoplanin expression was confirmed (V1 and V2, control stable clones 1 and 2 with empty vector; P4 and P15, stable clones 4 and 15 with podoplanin cDNA-inserted vector) C) The growth activity of each established clone indicated was examined as described in Methods. (N.S., no significant change versus V1). D) Migratory activities of each established clone were examined as described in Methods. No significant change in migratory activity was evident among EBC1 clones (N.S. versus V1). E) The phase contrast image of each cultured clone is shown. F) Cell lysates from the cultured stable clones indicated were subjected to Western blot analysis for phosphorylated ERM (p-ERM) molecules (p-Ezrin/Radixin and p-Moesin, arrows) and subsequently to re-probing for total ERM (Ezrin/Radixin and Moesin, arrowheads) and β-actin.

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Molecular Cancer

ISSN: 1476-4598