RGS transcript expression is decreased in multiple models of ovarian cancer chemoresistance. (A) RGS expression is decreased in cisplatin resistant A2780 cells. A hierarchical clustering heat map is shown depicting changes in RGS expression between parental A2780 cells (n = 5) and chemoresistant (resistant) A2780 cells (n = 5). As indicated in the side bar, red coloring represents a high level of comparative expression and green indicates a lower level (range 4.3 to -3.5). In the left panel, a box plot depicts the expression levels of multiple RGS transcripts in parental and drug resistant A2780 cells exposed to long-term cisplatin. RGS2, 5, 10, and 17 were significantly down regulated in repeated datasets and are presented here. Where multiple probes for the same gene were included on the microarray chip, these are distinguished with ('). *p < 0.05, ***p < 0.001 between groups, parental vs. chemoresistant cells. (B) RGS expression is decreased in vincristine-resistant SKOV-3 cells. A hierarchical clustering heat map is shown depicting changes in RGS expression between parental SKOV-3 cells (n = 2) and chemoresistant SKOV-3 cells (n = 2). In the left panel, a box plot depicts the expression levels of multiple RGS transcripts in parental (black bars) and vincristine resistant (open bars) SKOV-3 cells. (C) RGS expression is decreased in paclitaxel-resistant HeyA8 cells. RNA was isolated from parental and MDR-HeyA8 cells as described, and the expression of RGS2, RGS5, RGS10 and RGS17 transcripts was normalized to β2-microglobulin as an internal standard prior to the comparison between parental and multi-drug resistant HeyA8 cells. The fold change in expression relative to vehicle controls was calculated by the 2-ΔΔCt method. *p < 0.05, normalized control vs. RGS groups.