SKOV-3 cell sensitivity to cytotoxic drugs is altered by modulating RGS10 and RGS17 expression levels. (A) Transcript levels of RGS10 and RGS17 were determined using quantitative RT-PCR 72 hours after transient transfection with the indicated siRNA constructs. RGS transcript levels were normalized to GAPDH transcripts and reported relative to negative control-siRNA treated cells. (B) Overall cell viability was determined in SKOV-3 cells 48 hours following siRNA transfection in the absence of drug using CellTiter-Blue colorimetric cellular metabolism assays. (C) Cytotoxic drugs were added to cells at the indicated doses 24 hours after siRNA transfection, and cell viability was determined 48 hours after addition of drug. Data are shown normalized to cell viability in the absence of drug (100%). *: p < 0.05, **: p < 0.01,***: p < 0.001. (D-F) siRNA-mediated knock-down of RGS10 or RGS17 alone or in combination resulted in a right-shifted dose response curve of cisplatin (D), vincristine (E), or docetaxel (F) toxicity in SKOV-3 cells.