Figure 7

ZNF217 overexpression alters paclitaxel-induced apoptosis. (A) MDA-MB-231/pcDNA6, ZNF217-1 and ZNF217-2 cells were untreated (-) or treated with 10 or 100 nM paclitaxel, stained with Annexin-V-Fluos and propidium iodide and analyzed by flow cytometry (means ± s.d. from three independent experiments). **, P < 0.01 and ***, P < 0.001 versus the corresponding MDA-MB-231/pcDNA6 cells (Student's t-test). (B) MDA-MB-231/pcDNA6, ZNF217-1 and ZNF217-2 cells were treated with 100 nM paclitaxel. Caspase 3 activity was assessed (means ± s.d. from three independent experiments). ***, P < 0.001 versus MDA-MB-231/pcDNA6 cells (Student's t-test). (C) Western-blot analysis of PARP cleavage in response to 100 nM paclitaxel. Histogram represents quantification of the cleaved PARP in 100 nM paclitaxel-treated cells and tubulin expression was used for normalization (means ± s.d. of three independent experiments). ***, P < 0.001 versus paclitaxel-treated MDA-MB-231/pcDNA6 cells (Student's t-test). (D) Non-transfected (NT) or transfected MCF7 cells with either scrambled RNA or siRNA-B were treated with 100 nM paclitaxel. Caspase 3 activity was assessed (means ± s.d. from three independent experiments). *, P < 0.05 versus MCF7 cells transfected with scrambled RNA (Student's t-test).