Figure 2

Analyses of metabolism gene set regulated by oncogenic KRAS and HIF. A, heatmap analysis comparing the following metabolism gene sets: HCT116 vs HCT116^HIF-1α-/-, HCT116 vs HCT116^HIF-2α-/-, HCT116 vs HCT116^{HIF-1α-/-HIF-2α-/-}, and HCT116 vs HCT116^{WT KRAS} cells. Expression values are averages of three samples. B, Venn diagrams analyzing the extent of overlap of the following metabolism gene sets: 1) HCT116 vs HCT116^HIF-1α-/-, HCT116 vs HCT116^HIF-2α-/-, and HCT116 vs HCT116^{HIF-1α-/-HIF-2α-/-}; 2) HCT116 vs HCT116^{HIF-1α-/-HIF-2α-/-} and HCT116 vs HCT116^{WT KRAS}. Each circle represents a single gene set; and the number of genes common between the gene sets is denoted within the overlaps of the circles. C, Clonogenic survival assay of HCT116, HCT116^HIF-1α-/-, HCT116^HIF-2α-/-, HCT116^{HIF-1α-/-HIF-2α-/-}, and HCT116^{WT KRAS} cells. c, clone.