Figure 5

Effects of genetic inactivation of oncogenic KRAS or both HIF-1α and HIF-2α on mitochondrial metabolism. A, change in cellular O₂ consumption; B, change in MTT reduction; and C, change in intracellular ATP level in HCT116^{HIF-1α-/-HIF-2α-/-} and HCT116^{WT KRAS} relative to HCT116 cells (n = 5). Bars, stdev. p < 0.05 by Student's t test comparing knockout cells with parental HCT116 cells. D, cellular and mitochondrial ROS levels in HCT116, HCT116^{HIF-1α-/-HIF-2α-/-}, and HCT116^{WT KRAS} cells, as measured by the fluorescent dyes CM-H₂DCFDA and MitoSOX.