Figure 3

Inhibition of ER signaling decreases hMSC stimulated MCF-7 proliferation and migration in vitro. (A) MCF-7 cells were seeded in the lower chamber of a 24-well plate with MCF-7 cells or hMSCs were seeded in the upper well. Upper and lower chambers were treated with either DMSO (control) or ICI (100 nM). After 7 days of culture, inserts were removed and MTT was added to each well. Cells were solubilized and absorbance read. All data are represented as normalized percent proliferation compared to MCF-7 + DMSO (control) treated cells ± SEM, (* p < 0.05, ** p < 0.01). (B) MCF-7-GFP cells alone or in combination with hMSCs (1:1) were seeded in the upper chamber of a transwell system and lower wells contained culture media supplemented with 10% FBS (10%). Upper and lower chambers were treated with either DMSO (control) or ICI (100 nM). After 48 hours cells were fixed and the number of GFP positive migrated cells counted. Bars represent average number of migrated cell per condition ± SEM, (* p < 0.05, *** p < 0.001).