MAL mRNA expression in HNSCC specimens and HNSCC cell lines. (A) Representative results of gel electrophoresis from 24 pairs of HNSCC(C) and their adjacent normal oral epithelium (N). (B) Real-time RT-PCR analysis of MAL was carried out in 48 pairs of HNSCC and adjacent normal oral epithelium. For each sample, the relative mRNA level of MAL was normalized to β-actin. The line within each box represents the median Ct value; the upper and lower edges of each box represent the 75th and 25th percentiles, respectively; the upper and lower bars indicate the highest and lowest values determined, respectively. (C) Expression of MAL mRNA in nine representative HNSCC cell lines and human immortalized oral epithelial cells (HIOEC) using semi-quantitative RT-PCR analysis with normal human oral epithelial cell (NHOEC) as a positive control (POS.) and no cDNA template as a negative control (NEG.).