Sigma-2 ligands induce generation of reactive oxygen species in Panc02 cells. A. Sigma-2 ligands induce oxidation of 5-(and-6)-carboxy-2',7'-dichlorodihydro-fluorescein diacetate (carboxy-H2DCFDA) to 2',7'-dichlorofluorescein (DCF) and quantified by fluorescence of DCF. B. Viability was partially rescued for SW43 and siramesine (SRM) in the presence of alpha-tocopherol (200 μg/mL), but not for SV119. C. Sigma-2 ligand induced caspase-3 activity was partially reduced for SW43 in the presence of lipophilic α-toco, but completely for SRM. D. Flow cytometry for Annexin-V verifies that caspase-3 activity correlates with apoptosis. (Means ± SEM), n ≥ 3, *p < 0.05, **p > 0.05.