Figure 4
From: Luciferase expression and bioluminescence does not affect tumor cell growth in vitro or in vivo
The luciferase gene or luciferase bioluminescence does not affect the growth of tumors in mice. B16-F10 cells (1 × 106) expressing GFP alone or GFP-P2A-luc (clone 4) were injected subcutaneously into the right or left flank respectively of white C57Bl/6 mice. Mice received bi-weekly injections of either PBS or D-luciferin (150 mg/kg) and tumor growth was recorded using calipers for GFP alone (A) or GFP-P2A-luc (B). Ellipsoid volume was determined using the formula π/6 × (L × W)3/2 [21]. After 21 days, bioluminescent imaging was performed on tumors expressing GFP-P2A-luc (C) using the Xenogen IVIS-100. (D) Images were analyzed using Living Image software and represented as total flux measurements in photons/second. (E) Weight data was recorded from the excised tumors at sacrifice day 21. Statistical significance was assessed using non-parametric (Mann-Whitney) analysis using GraphPad Prism 5.01. Data is the mean ± s.e.m. from 4 independent experiments.