Figure 2

The PSAP expression correlated with migratory and invasive potential of prostate cancer cell lines. (A) Transwell filter migration and invasion assays. Stable PSAP-KD clones of PC-3 and DU-145 were seeded on transwell filters and incubated for 24 h. Basal medium containing 5% FBS was used as chemo-attractant. For the invasion assay, the membrane was pre-coated with 50 μg Matrigel. (B) Cells migrated or invaded were counted from ten random fields at 100× magnification using a phase-contrast microscope. (C) Effects of rhPSAP on cell migration and invasion. A representative control and PSAP-KD clone from each cell line was seeded on transwell filters and incubated for 24 h (migration) or 48 h (invasion). As a chemo-attractant, basal medium containing 0.5% FBS and various amount of rhPSAP (0, 1, 10, 50 nM) were included in the lower compartment of the transwell filters. Each bar represented the mean ± SEM of three independent experiments, each in quadruplicates. ANOVA was used to examine the significance of the data (p < 0.0001) comparing the PSAP-KD clones relative to control clones in each cell line or among different treatment concentrations for rhPSAP and control.