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Figure 2 | Molecular Cancer

Figure 2

From: Epigenetic control of the basal-like gene expression profile via Interleukin-6 in breast cancer cells

Figure 2

Reduced methylation at IL-6prox and induction of IL-6 expression by exogenous or autocrine IL-6. a) IL-6 ELISA assay, RT-PCR analysis of IL-6 mRNA level and quantitative evaluation of IL-6prox MS-PCR analysis in MCF-7 cells in presence/absence of IL-6 (10 ng/ml, 48 h) or 5AzadCytidine (5azadC, 15 μM, 48 h); b) IL-6 ELISA assay, RT-PCR analysis of IL-6 mRNA level, quantitative evaluation of IL-6prox MS-PCR analysis and IL-6 promoter driven luciferase assay (IL6P-Luc) in MDA-MB231cells and p53 D cells in presence/absence to αIL-6 (1.5 μg/ml, 48 h); C) IL-6 ELISA assay, RT-PCR analysis of IL-6 mRNA level and quantitative evaluation of IL-6prox MS-PCR analysis in MCF-7 derived mammospheres (MCF-7S) or MCF-7 adherent cells; β-Actin was assessed as quantitative control for RT-PCR analysis. Student t test, *p < 0.05; #p < 0.005. n.a.: not assessed.

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Molecular Cancer

ISSN: 1476-4598