Figure 6
Gain of ERα promoter ( ERαp ) methylation by autocrine or exogenous IL-6. Quantitative evaluation of ERαp MS-PCR analysis in: a) MCF-7 cells in presence/absence of IL-6 (10 ng/ml, 48 h) or 5azadC (15 μM, 48 h); b) p53 D and MDA-MB231 cells in presence/absence of αIL-6 (1.5 μg/ml, 48 h) or scr/siIL-6 (1 μg, 48 h). PD: Primer Dimers, NSF Non Specific Fragment.