Figure 7
IL-6dist gains methylation in response to IL-6 and contains putative repressive IRF-1/IRF-2 binding sites. Quantitative evaluation of IL-6dist MS-PCR analysis in: a) MCF-7 cells in presence/absence of IL-6 (10 ng/ml, 48 h) or 5azadC (15 μM, 48 h), b) p53 D and MDA-MB231cells in presence/absence of αIL-6 (1.5 μg/ml, 48 h); c) Luciferase assay of MCF-7 cells transfected with IL6P-Luc in presence/absence of IL-6 (10 ng/ml), and pIRF-1 or pIRF-2 (1 μg each, 24/48 h) in presence/absence of IL-6 (10 ng/ml, lower panel). Student t test, *p < 0.05; #p < 0.005.