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Figure 8 | Molecular Cancer

Figure 8

From: Epigenetic control of the basal-like gene expression profile via Interleukin-6 in breast cancer cells

Figure 8

CD133p2 gains methylation in response to IL-6 and contains putative repressive ERα binding sites. Quantitative evaluation of CD133 p2 MS-PCR analysis in: a) MCF-7 cells in presence/absence of IL-6 or 5azadC (10 ng/ml and 15 μM, 48 h, respectively), b) p53 D and MDA-MB231 cells in presence/absence of αIL-6 (1.5 μg/ml, 48 h); c) RT-PCR analysis of ERα and CD133 mRNA level and quantitative evaluation of CD133p2 MS-PCR analysis in MCF-7 cells exposed to Tamoxifen (4OHT, 3 μM, 48 h or vehicle) or to scr or ERα specific siRNA (siERα, 1 μg, 48 h); β-Actin was assessed as quantitative control in RT-PCR analysis. PD: primer Dimers.

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